Among the 3 primary habitats regarding the biosphere, the marine realm which covers 70% regarding the earths surface sends the largest inhabitable space for living organisms. The read dishwasher baby bottle basket diversity is important sequential to understand the community structure and pattern of distribution Surajit Das et al 2006. For many years, the filamentous blue-green algae cyanobacteria were believed to be primarily responsible for N2 fixation in oceanic waters due to the fact that little or negligible in situ rates were observed in their absence and there was a correlation of in situ N2 fixation with light intensity. However, evidence was accumulating which documents the importance of bacterial N2 fixations in many and diverse marine habitats MARY LOU GUERINOT et al 1985. It is commonly assumed that marine bacteria, since they live within the sea, should be Salt-tolerant organisms.
ZoBell and Upham define marine bacteria as being bacteria from the sea which on initial isolation compulsory seawater within the moderate for growth. That is why analysis of marine h2o shall give the effect of salts on the growth of marine Azotobacter. Biofertilizers are the source of microbial inoculants, which have brought hopes for many countries most economically and environmentally. Azotobacter sp is free living, known to fix atmospheric nitrogen. There exists different strains of Azotobacter each has varied chemical, biological and other characters.
Azotobacter and Azospirillum are 3 other efficient bacteria. The response of these organisms in increasing crop yield was commonly experienced. These are the biofertilizers within the cultivation of most crops. Inoculation of soil or seed with Azotobacter is effective in increasing yields of crops in well-manured soil with high organic reason content. Experiments with Azotobacter cultures and crop plants at the Indian Agricultural Studies Institute, New Delhi, lead us to know that significant increases in growth and yield of wheat, pasta and greens crops should be obtained in pot trials.
However, below field conditions, such uniform trends towards increases in yield are not always reproducible. We carried out pot lifestyle experiment sequential to assess the effects of Azotobacter isolated from marine source on the growth of Black gram. Their shoot length, root length and their chlorophyll content were measured. MATERIALS AND METHOD: Sample collection: Samples of surface h2o were collected within the region of Thundi region Palk Bay. Sample collection was accomplished at the interval of approximately 20 days Surface h2o samples at depths of 1-2 m were collected in sterile tube containing Azotobacter selective medium.
Sediment samples were collected separately in broth medium. two and 3 Chemical parameter of sea water: Collected h2o samples were analyzed for total hardness i. e the presence of magnesium and calcium by EDTA 0. 01 M Ethylene diamine tetra acetic acid titration method. Total Chlorine content was analyzed by Mohr method.
In EDTA method 60 ml of h2o sample was pipetted to an Erlenmeyer flask. About 2ml of buffer solution mix of ammonium chloride and ammonium hydroxide, was added to sample. A little drops of indicator Eriochrome black were added and the solution was gently stried. The EDTA solution was taken within the burette and titrated with h2o sample until the color regarding the solution turns dark brown to purple to blue. As soon as the color regarding the solution turned blue, stopped the titration and record the final position of EDTA solution within the burette.
Finally the experimental concentration of calcium and magnesium ions within the unknown h2o sample was calculated. The hardness of h2o sample should be classified creating use of a sum of all the calcium and magnesium ions in solution. In Mohr method 20 ml of sodium chloride 0. 01 M solution was pipette in 250 ml Erlenmeyer flask. Approx 2ml of dipotassium chromate indicator was added to solution.
Solution was turned bright yellow color. 01 M solution was taken within the burette. The known chloride was titrated with silver nitrate until the color changed from bright yellow to brick dark brown color swirl the flask constantly to look the uniform color. Finally the experimental concentration of chloride within the known solution was calculated. To determine unknown chloride, six ml of h2o sample was taken in 250 ml Erlenmeyer flask.
2ml of indicator dipotassium chromate was added. 01 M solution was taken within the burette. The h2o sample was carefully titrated with silver nitrate solution. Near the end spot drop by drop was added from the burette as soon as the color regarding the solution turned yellow to red, stopped the reaction and recorded the final position of silver nitrate solution within the burette. Finally the experimental concentration of chloride within the unknown solution was calculated.
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